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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-15566 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal beta 2-Microglobulin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2-Microglobulin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse plasma (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:1500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: beta 2-Microglobulin Antibody [NBP2-15566] - HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of B2M protein by 4 ug beta 2 Microglobulin antibody 15 % SDS-PAGE The mmunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Paraffin-embedded human endometrial cancer. beta 2 Microglobulin antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Paraffin-embedded Cal27 xenograft. beta 2 Microglobulin antibody dilution: 1:500.
