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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX112815 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112815, RRID:AB_11175135
- Product name
- beta 2 Microglobulin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Evaluation of immune-mediated idiosyncratic drug toxicity using chimeric HLA transgenic mice.
Susukida T, Aoki S, Kogo K, Fujimori S, Song B, Liu C, Sekine S, Ito K
Archives of toxicology 2018 Mar;92(3):1177-1188
Archives of toxicology 2018 Mar;92(3):1177-1188
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE GTX112815 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 £gg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 £gg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- beta 2 Microglobulin antibody immunoprecipitates B2M protein in IP experiments.IP samples: HeLa whole cell extractA. 30 £gg HeLa whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of B2M protein by 4 £gg beta 2 Microglobulin antibody (GTX112815)15 % SDS-PAGEThe immunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- beta 2 Microglobulin antibody detects B2M protein at cytosol on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. beta 2 Microglobulin antibody (GTX112815) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- beta 2 Microglobulin antibody detects beta 2 Microglobulin protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
