R1065P

antibody from Acris Antibodies GmbH

Targeting: B2M

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1065P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1065P, RRID:AB_973248

Product name

anti Beta-2-microglobulin

Antibody type

Polyclonal

Antigen

beta-2-Microglobulin [Human Urine].

Reactivity

Human

Host

Rabbit

Vial size

0.5 mg

Concentration

5.0 mg/ml (by UV absorbance at 280 nm)

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry (Formalin/PFA-Fixed Paraffin Embedded Sections): R1065P Beta-2-Microglobulin antibody staining of normal Human skin. Staining is localized to cell membrane (and to lesser amount cytoplasmatic compartment).Right Panel: with primary antibody at 1 µg/ml.Left Panel: Isotype Control. Sections were stained using an automated system DAKO Autostainer Plus, at RT. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counter-stained with Haematoxylin and coverslipped under DePeX. For manual staining it is recommended to optimize conditions for primary antibody concentration and incubation time (overnight); amplification may be required.