Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-47704APC - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal beta 2-Microglobulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): beta 2-Microglobulin Antibody (B2M/961) [Allophycocyanin] [NBP2-47704APC] - A surface stain was performed on hPBMCs with beta 2-Microglobulin antibody (B2M/961) NBP2-47704APC (blue) and a matched isotype control NBP2-27228APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.