ABIN458094

antibody from antibodies-online

Targeting: B2M

No application information provided.

Antibody Data

Product number

ABIN458094

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN458094, RRID:AB_10780593

Product name

anti-beta-2-Microglobulin (B2M) antibody

Antibody type

Polyclonal

Antigen

Beta-2 microglobulin is a member of the Immunoglobulin superfamily. The amino acid sequence resembles that of immunoglobulin constant domains. It is isolated from urine of patients with impaired readsorption in the kidney tubules. High levels of beta-2 microglobulin are as present in cord blood serum and foetal serum. The host animal is immunised with highly purified beta-2 microglobulin. Freund?s complete adjuvant is used in the first step of the immunization procedure.

Description

Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

1 mL

Concentration

Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against appropriate serially diluted antigens in agar-b

Storage

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.