316302

antibody from BioLegend

Targeting: B2M

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

316302

Provider

BioLegend

Proper citation

BioLegend Cat#316302, RRID:AB_492835

Product name

Purified anti-human ‰2-microglobulin

Antibody type

Monoclonal

Antigen

Purified human β2-microglobulin

Description

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

Reactivity

Human, Porcine

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

NLRC5 cooperates with the RFX transcription factor complex to induce MHC class I gene expression.

Meissner TB, Liu YJ, Lee KH, Li A, Biswas A, van Eggermond MC, van den Elsen PJ, Kobayashi KS
Journal of immunology (Baltimore, Md. : 1950) 2012 May 15;188(10):4951-8

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Distinct functions for the glycans of tapasin and heavy chains in the assembly of MHC class I molecules.

Rizvi SM, Del Cid N, Lybarger L, Raghavan M
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2309-20

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained with purified 2M2, followed by anti-mouse FITC

Conjugate

Unconjugated