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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [2]
- Immunocytochemistry [1]
- Flow cytometry [4]
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- Product number
- NBP1-44520 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-44520, RRID:AB_10007961
- Product name
- Mouse Monoclonal beta 2-Microglobulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
- Reactivity
- Human, Mouse, Bovine, Canine, Chicken/Avian, Rabbit
- Host
- Mouse
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C in the dark.
Submitted references Defining CD8+ T cell determinants during human viral infection in populations of Asian ethnicity.
Rivino L, Tan AT, Chia A, Kumaran EA, Grotenbreg GM, MacAry PA, Bertoletti A
Journal of immunology (Baltimore, Md. : 1950) 2013 Oct 15;191(8):4010-9
Journal of immunology (Baltimore, Md. : 1950) 2013 Oct 15;191(8):4010-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human beta2-microglobulin (B2M-01). Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Analysis of HLA-G and beta2-microglobulin in supernatant of HLA-G transfectants with anti-beta2-microglobulin (B2M-01) HRP as the detection antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Human serum using anti-HLA-G (capture) and anti-beta2 microglobulin HRP (detection).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Confocal microscopy of human b2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor 647 conjugate and B) with Alexa Fluor 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) biotin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PerCP-CyTM5.5.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PE.
