MA5-42598

antibody from Invitrogen Antibodies

Targeting: CTBP2 ribeye

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42598

Provider

Invitrogen Antibodies

Product name

CtBP2 Recombinant Rabbit Monoclonal Antibody (1R1U6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive test controls include: HeLa, NIH/3T3, Rat testis. The target is usually found in the following locations: Cell junction, Nucleus, synapse. Immunogen sequence: TGRIPESLRN CVNKEFFVTS APWSVIDQQA IHPELNGATY RYPPGIVGVA PGGLPAAMEG IIPGGIPVTH NLPTVAHPSQ APSPNQPTKH GDNREHPNEQ

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

1R1U6

Vial size

100 µL

Concentration

0.33 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CtBP2 in NIH/3T3 cells using a CtBP2 Recombinant Monoclonal antibody (Product # MA5-42598). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CtBP2 in NIH/3T3 cells using a CtBP2 Recombinant Monoclonal antibody (Product # MA5-42598). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CtBP2 in NIH/3T3 cells using a CtBP2 Recombinant Monoclonal antibody (Product # MA5-42598). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CtBP2 in NIH/3T3 cells using a CtBP2 Recombinant Monoclonal antibody (Product # MA5-42598). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CtBP2 in NIH/3T3 cells using a CtBP2 Recombinant Monoclonal antibody (Product # MA5-42598). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CtBP2 in NIH/3T3 cells using a CtBP2 Recombinant Monoclonal antibody (Product # MA5-42598). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CtBP2 in NIH/3T3 cells. Samples were incubated with CtBP2 Monoclonal antibody (Product # MA5-42598) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CtBP2 in PC-12 cells. Samples were incubated with CtBP2 Monoclonal antibody (Product # MA5-42598) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CtBP2 in U2OS cells. Samples were incubated with CtBP2 Monoclonal antibody (Product # MA5-42598) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CtBP2 in 300 μg extracts of Hela cells. Samples were precipitated with 3 μg CtBP2 Monoclonal antibody (Product # MA5-42598). Western blot was performed from the immunoprecipitate using CtBP2 Monoclonal antibody (Product # MA5-42598) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CtBP2 in paraffin-embedded human colon carcinoma. Samples were incubated with CtBP2 Monoclonal antibody (Product # MA5-42598) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CtBP2 in paraffin-embedded mouse kidney. Samples were incubated with CtBP2 Monoclonal antibody (Product # MA5-42598) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CtBP2 in paraffin-embedded rat ovary. Samples were incubated with CtBP2 Monoclonal antibody (Product # MA5-42598) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.