HPA007928

antibody from Atlas Antibodies

Targeting: GPRC5A PEIG-1, RAI3, RAIG1, TIG1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA007928

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA007928, RRID:AB_1849318

Product name

Anti-GPRC5A

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human GPRC5A, Gene description: G protein-coupled receptor, class C, group 5, member A, Alternative Gene Names: GPCR5A, RAI3, RAIG1, Validated applications: WB, IHC, ICC, Uniprot ID: Q8NFJ5, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

GPRC5A promotes lung colonization of esophageal squamous cell carcinoma

Zhou H, Tan L, Zhang B, Kwong D, Wong C, Zhang Y, Ru B, Lyu Y, Siu K, Luo J, Yang Y, Liu Q, Chen Y, Zhang W, He C, Jiang P, Qin Y, Liu B, Guan X
Nature Communications 2024;15(1)

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Crystalline silica particles cause rapid NLRP3-dependent mitochondrial depolarization and DNA damage in airway epithelial cells

Wu R, Högberg J, Adner M, Ramos-Ramírez P, Stenius U, Zheng H
Particle and Fibre Toxicology 2020;17(1)

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Adaptive RSK‐EphA2‐GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancer

Moyano‐Galceran L, Pietilä E, Turunen S, Corvigno S, Hjerpe E, Bulanova D, Joneborg U, Alkasalias T, Miki Y, Yashiro M, Chernenko A, Jukonen J, Singh M, Dahlstrand H, Carlson J, Lehti K
EMBO Molecular Medicine 2020;12(4)

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RhoA/C inhibits proliferation by inducing the synthesis of GPRC5A

Richter L, Oberländer V, Schmidt G
Scientific Reports 2020;10(1)

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Single-cell transcriptomics reveals multi-step adaptations to endocrine therapy

Hong S, Chan T, Lombardo Y, Corleone G, Rotmensz N, Bravaccini S, Rocca A, Pruneri G, McEwen K, Coombes R, Barozzi I, Magnani L
Nature Communications 2019;10(1)

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GPCRomics: GPCR Expression in Cancer Cells and Tumors Identifies New, Potential Biomarkers and Therapeutic Targets

Insel P, Sriram K, Wiley S, Wilderman A, Katakia T, McCann T, Yokouchi H, Zhang L, Corriden R, Liu D, Feigin M, French R, Lowy A, Murray F
Frontiers in Pharmacology 2018;9

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Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesion

Bulanova D, Akimov Y, Rokka A, Laajala T, Aittokallio T, Kouvonen P, Pellinen T, Kuznetsov S
Cell Adhesion & Migration 2017;11(5-6):434-446

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GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR

Zhou H, Telonis A, Jing Y, Xia N, Biederman L, Jimbo M, Blanco F, Londin E, Brody J, Rigoutsos I
Cell Death & Disease 2016;7(7):e2294-e2294

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & vesicles.

Sample type

Human

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human lung and skeletal muscle tissues using HPA007928 antibody. Corresponding GPRC5A RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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