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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P49BT-25UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thrombopoietin Polyclonal Antibody, Biotin, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TPO. Anti-Human TPO-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TPO (500-P49) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P49BT-1MG will be provided as 2 x 500 µg
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 25 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Analysis of Jak2 signaling reveals resistance of mouse embryonic hematopoietic stem cells to myeloproliferative disease mutation.
Mascarenhas MI, Bacon WA, Kapeni C, Fitch SR, Kimber G, Cheng SW, Li J, Green AR, Ottersbach K
Blood 2016 May 12;127(19):2298-309
Blood 2016 May 12;127(19):2298-309
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect hTPO by Western Blot analysis Thrombopoietin Polyclonal Antibody, Biotin (Product # 500-P49BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect hTPO by Western Blot analysis Thrombopoietin Polyclonal Antibody, Biotin (Product # 500-P49BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Thrombopoietin Polyclonal Antibody, Biotin (Product # 500-P49BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Thrombopoietin Polyclonal Antibody (Product # 500-P49-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTPO.
- Conjugate
- Biotin
