Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- PAB7067 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7067, RRID:AB_1575287
- Product name
- GPX7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of GPX7.
- Antigen sequence
CNQFGQQEPDSNK
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Glutathione-dependent and -independent oxidative stress-control mechanisms distinguish normal human mammary epithelial cell subsets.
Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells.
Kannan N, Nguyen LV, Makarem M, Dong Y, Shih K, Eirew P, Raouf A, Emerman JT, Eaves CJ
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):7789-94
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):7789-94
Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells.
Utomo A, Jiang X, Furuta S, Yun J, Levin DS, Wang YC, Desai KV, Green JE, Chen PL, Lee WH
The Journal of biological chemistry 2004 Oct 15;279(42):43522-9
The Journal of biological chemistry 2004 Oct 15;279(42):43522-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GPX7 polyclonal antibody (Cat # PAB7067) (0.3 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GPX7 polyclonal antibody (Cat # PAB7067) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL).
- Validation comment
- Sandwich ELISA