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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-21681 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPX7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Glutathione peroxidase 7 tansfected 293T cell.
- Concentration
- 0.52 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPX7 was performed by separating of whole cell lysates by 12% SDS PAGE. Proteins were transferred to a membrane and probed with a GPX7 Polyclonal Antibody (Product # PA5-21681) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A: 293T cell line non-transfected lysate. , B: 293T cell line GPX7 transfected lysate. .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPX7 Polyclonal Antibody detects GPX7 protein at cytoplasm on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. GPX7 Polyclonal Antibody (Product # PA5-21681) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H441 xenograft, using GPX7 (Product # PA5-21681) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
