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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-28854 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPX7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: Mouse embryonic fibroblast.
- Concentration
- 0.26 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GPX7 Polyclonal Antibody (Product # PA5-28854). Sample (20 µg of whole cell lysate). A: Mouse embryonic fibroblast (WT). B: Mouse embryonic fibroblast (Gpx7 -/-) . 12% SDS PAGE. GPX7 Polyclonal Antibody (Product # PA5-28854) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPX7 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a GPX7 Polyclonal Antibody (Product # PA5-28854) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GPX7 Polyclonal Antibody (Product # PA5-28854). Sample (20 µg of whole cell lysate). A: Mouse embryonic fibroblast (WT). B: Mouse embryonic fibroblast (Gpx7 -/-) . 12% SDS PAGE. GPX7 Polyclonal Antibody (Product # PA5-28854) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPX7 Polyclonal Antibody detects GPX7 protein at cytoplasm on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. GPX7 Polyclonal Antibody (Product # PA5-28854) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
