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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA1-23306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPX7 Monoclonal Antibody (2704)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Recommended positive controls: HeLa whole cell extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2704
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Transcript and protein analysis reveals better survival skills of monocyte-derived dendritic cells compared to monocytes during oxidative stress.
Van Brussel I, Schrijvers DM, Martinet W, Pintelon I, Deschacht M, Schnorbusch K, Maes L, Bosmans JM, Vrints CJ, Adriaensen D, Cos P, Bult H
PloS one 2012;7(8):e43357
PloS one 2012;7(8):e43357
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPX7 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a GPX7 Monoclonal Antibody (2704) (Product # MA1-23306) at a dilution of 1:500.The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GPX7 Monoclonal Antibody (2704) (Product # MA1-23306). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GPX7 Monoclonal Antibody (2704) (Product # MA1-23306) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GPX7 Monoclonal Antibody (2704) (Product # MA1-23306). Detection of GPX7 using GPX7 Monoclonal Antibody (2704) (Product # MA1-23306) in HeLa whole cell extract.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis using GPX7 Monoclonal Antibody (2704) (Product # MA1-23306).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPX7 Monoclonal Antibody (2704) detects GPX7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. GPX7 stained by GPX7 Monoclonal Antibody (2704) (Product # MA1-23306) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
