H00114609-D01

antibody from Abnova Corporation

Targeting: TIRAP Mal, wyatt

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00114609-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00114609-D01, RRID:AB_10719395

Product name

TIRAP MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human TIRAP protein.

Antigen sequence

MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKK
RPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLP
PTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVS
YLEGSTASLRCFLQLRDATPGGAIVSELCQALSSS
HCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCT
IPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVK
EAVMRYLQTLSWHLLYHGTPEIGVKLETENPCRAS
DSHKCDKRYRE


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Phosphatidylinositol 4-phosphate 5-kinase α facilitates Toll-like receptor 4-mediated microglial inflammation through regulation of the Toll/interleukin-1 receptor domain-containing adaptor protein (TIRAP) location.

Nguyen TT, Kim YM, Kim TD, Le OT, Kim JJ, Kang HC, Hasegawa H, Kanaho Y, Jou I, Lee SY
The Journal of biological chemistry 2013 Feb 22;288(8):5645-59

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TIRAP expression in transfected 293T cell line (H00114609-T01) by TIRAP MaxPab polyclonal antibody.Lane 1: TIRAP transfected lysate(28.27 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of TIRAP transfected lysate using anti-TIRAP MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TIRAP MaxPab mouse polyclonal antibody (B01) (H00114609-B01).

Validation comment

Immunoprecipitation

Protocol

Protocol