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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 19182-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#19182-1-AP, RRID:AB_10603471
- Product name
- SCP2/SCPx antibody
- Antibody type
- Polyclonal
- Description
- SCP2/SCPx antibody (Cat. #19182-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Membrane topologies of PEX13 and PEX14 provide new insights on the mechanism of protein import into peroxisomes.
Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays.
The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo.
A cargo-centered perspective on the PEX5 receptor-mediated peroxisomal protein import pathway.
Barros-Barbosa A, Ferreira MJ, Rodrigues TA, Pedrosa AG, Grou CP, Pinto MP, Fransen M, Francisco T, Azevedo JE
The FEBS journal 2019 Jan;286(1):205-222
The FEBS journal 2019 Jan;286(1):205-222
Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays.
Francisco T, Dias AF, Pedrosa AG, Grou CP, Rodrigues TA, Azevedo JE
Methods in molecular biology (Clifton, N.J.) 2017;1595:27-35
Methods in molecular biology (Clifton, N.J.) 2017;1595:27-35
The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo.
Dias AF, Rodrigues TA, Pedrosa AG, Barros-Barbosa A, Francisco T, Azevedo JE
The Journal of biological chemistry 2017 Sep 15;292(37):15287-15300
The Journal of biological chemistry 2017 Sep 15;292(37):15287-15300
A cargo-centered perspective on the PEX5 receptor-mediated peroxisomal protein import pathway.
Francisco T, Rodrigues TA, Freitas MO, Grou CP, Carvalho AF, Sá-Miranda C, Pinto MP, Azevedo JE
The Journal of biological chemistry 2013 Oct 4;288(40):29151-9
The Journal of biological chemistry 2013 Oct 4;288(40):29151-9
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19182-1-AP(SCPX antibody) at dilution of 1:2000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SCP2 antibody 19182-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
