Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-87810 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87810, RRID:AB_11017780
- Product name
- Rabbit Polyclonal nSMase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human nSMase antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Integrated analysis of proteomics and metabolomics reveals the potential sex determination mechanism in Odontobutis potamophila.
Wang T, Zhu W, Zhang H, Wen X, Yin S, Jia Y
Journal of proteomics 2019 Sep 30;208:103482
Journal of proteomics 2019 Sep 30;208:103482
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: nSMase Antibody [NBP1-87810] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human skeletal muscle shows very weak positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.