Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B10767 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ PPP3CB Antibody (aa63-393) LS-B10767
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. PPP3CB antibody diluted at 1:1000.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug whole cell lysate). A: A431, B: MOLT4 . 7.5% SDS PAGE. PPP3CB antibody diluted at 1:1000
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. PPP3CB antibody diluted at 1:1000.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- PPP3CB antibody detects PPP3CB protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. PPP3CB protein stained by PPP3CB antibody diluted at 1:500.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- PPP3CB antibody detects PPP3CB protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. PPP3CB protein stained by PPP3CB antibody diluted at 1:500.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- PPP3CB antibody detects PPP3CB protein at cytoplasm by immunofluorescent analysis. HeLa cells were fixed in ice-cold MeOH for 5 min. PPP3CB protein stained by PPP3CB antibody diluted at 1:500.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-PPP3CB antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.