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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-22148 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP3CB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, Molt-4, mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PPP3CB was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PPP3CB Polyclonal Antibody (Product # PA5-22148) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PPP3CB Polyclonal Antibody (Product # PA5-22148). Sample (30 µg of whole cell lysate). Lane A: Molt-4 . 7.5% SDS PAGE. PPP3CB Polyclonal Antibody (Product # PA5-22148) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PPP3CB Polyclonal Antibody (Product # PA5-22148). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. PPP3CB Polyclonal Antibody (Product # PA5-22148) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using PPP3CB (Product # PA5-22148) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
