PA5-75664

antibody from Invitrogen Antibodies

Targeting: HCAR1 FKSG80, GPR104, GPR81, HCA1, LACR1, TA-GPCR

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-75664

Provider

Invitrogen Antibodies

Product name

GPR81 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice.

Chen S, Zhou L, Sun J, Qu Y, Chen M
Frontiers in physiology 2021;12:709135

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GPR81 in Lane 1: HEK293T cell lysate, Lane 2: Raw264.7 cell lysate, Lane 3: PC12 cell lysate. Samples were incubated with GPR81 polyclonal antibody (Product # PA5-75664) at a dilution of 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Western blot analysis and relative fold protein expression of GPR81, P-CREB, CREB, and the ratios of P-PKA/PKA and P-CREB/CREB after chronic administration. (A) Western blot analysis of GPR81 and proteins involved in cAMP-PKA pathway. (B) Fold protein expression of GPR81. (C) The ratio of P-PKA/PKA. (D) Fold protein expression of P-CREB. (E) Fold protein expression of CREB. (F) The ratio of P-CREB/CREB. Three bands are used for statistics. The data are presented as the mean+-SD, and significant differences among the groups were analyzed with one-way ANOVA. * p