Explore
Validate
LearnAHR-012-200UL
antibody from Invitrogen Antibodies
Targeting: HCAR2
GPR109A, HCA2, HM74A, NIACR1, Puma-g, PUMAG
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AHR-012-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR109A/HCAR2 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat spleen lysates (lanes 1 and 3) and rat colon membranes (lanes 2 and 4): - 1-2. Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (1:200). 3-4. Anti-GPR109A/HCAR2 (extracellular) Antibody , preincubated with GPR109A/HCAR2 (extracellular) Blocking Peptide (BLP-HR012).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 monocytic leukemia cell line lysates (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysates (lanes 2 and 4): - 1-2. Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (1:200). 3-4. Anti-GPR109A/HCAR2 (extracellular) Antibody , preincubated with GPR109A/HCAR2 (extracellular) Blocking Peptide (BLP-HR012).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR109A in rat cingulate cortex. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. GPR109A immunoreactivity (green) appears in outlines of cortical neurons (arrows). B. Pre-incubation of the Antibody with GPR109A/HCAR2 (extracellular) Blocking Peptide (BLP-HR012), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of GPR109A by indirect flow cytometry in live intact mouse J774 macrophage cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti- GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (2.5μg) + goat- Anti-rabbit-FITC.
