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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- FAB2760A - Provider product page
- Provider
- R&D Systems
- Product name
- Human HM74A/PUMA-G/GPR109A APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human HM74A/PUMA-G/GPR109A. Stains human HM74A/PUMA-G/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Red dye
- Antigen sequence
Q8TDS4- Isotype
- IgG
- Antibody clone number
- 245106
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry. Human peripheral blood neutrophils were stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HM74A/PUMA-G/GPR109A in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human HM74A/PUMA-G/GPR109A or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
