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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [2]
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- Product number
- MAB2760 - Provider product page
- Provider
- R&D Systems
- Product name
- Human HM74A/PUMA-G/GPR109A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human HM74A/GPR109A. Stains human HM74A/GPR109A-transfected cells but not irrelevant transfectants. Cross-reactivity with HM74 (GPR109B) was not tested.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q8TDS4- Isotype
- IgG
- Antibody clone number
- 245106
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Activation of Gpr109a, receptor for niacin and the commensal metabolite butyrate, suppresses colonic inflammation and carcinogenesis.
Neutrophil apoptosis mediated by nicotinic acid receptors (GPR109A).
Singh N, Gurav A, Sivaprakasam S, Brady E, Padia R, Shi H, Thangaraju M, Prasad PD, Manicassamy S, Munn DH, Lee JR, Offermanns S, Ganapathy V
Immunity 2014 Jan 16;40(1):128-39
Immunity 2014 Jan 16;40(1):128-39
Neutrophil apoptosis mediated by nicotinic acid receptors (GPR109A).
Kostylina G, Simon D, Fey MF, Yousefi S, Simon HU
Cell death and differentiation 2008 Jan;15(1):134-42
Cell death and differentiation 2008 Jan;15(1):134-42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HM74A/PUMA-G/GPR109A in Human Blood Neutrophils by Flow Cytometry. Human peripheral blood neutrophils were stained with (A) Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB2760) or (B) isotype control antibody (Catalog # MAB0061) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Mouse anti-Human Fc gamma RIII (CD16) PE-conjugated Monoclonal Antibody (Catalog # FAB2546P. View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HM74A/PUMA-G/GPR109A in HEK293 Human Cell Line Transfected with Human HM74A/PUMA-G/GPR109A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human HM74A/PUMA-G/GPR109A or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB2760) followed by anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins.
