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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- 102-PA09S - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- Anti-human Amphiregulin
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- antibody Protein-A purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
MSVRVEQVVK PPQNKTESEN TSDKPKRKKK GG
KNGKNRRN RKKKNPCNAE FQNFCIHGEC KYIE
HLEAVT CKCQQEYFGE RCGEKSMKTH SMIDSS
LSKL EHHHHHH- Vial size
- 100 µg
- Storage
- The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human Amphiregulin using a polyclonal rabbit anti-human Amphiregulin antibody [Cat# 102-PA09].
- Sample type
- Purified Protein
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- IF staining of human Amphiregulin in a mixture of primary human dermal lymphatic/umbilical vein endothelial cells (HDLEC/HUVEC) and primary normal human dermal fibroblasts (NHDF) with a polyclonal rabbit anti-human CD31 antibody (Protein-A purified). Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour (1:600) [Dianova].
- Sample type
- primary cells
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human Amphiregulin (5µg/ml) [Cat# 102-PA09] and counter staining of nuclei with Dapi. [400X] The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.
- Sample type
- cryo-sections of vasa vasorum of a human lymphatic collector
