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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- RQ4538 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Amphiregulin Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific Amphiregulin antibody is suitable for use in Western blot/Direct ELISA applications with mouse and rat samples.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Amphiregulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain and 2) mouse brain lysate with Amphiregulin antibody at 0.5ug/ml. Molecular weight: 28 kDa (non-glycosylated), ~50 kDa (glycosylated pro form), ~43 kDa (predominant glycosylated soluble form) as well as other, smaller, soluble and membrane bound forms.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) rat thymus, 3) rat spleen, 4) rat PC-12, 5) mouse brain, 6) mouse thymus, 7) mouse spleen and 8) mouse RAW264.7 cell lysate with Amphiregulin antibody at 0.5ug/ml. Molecular weight: 28 kDa (non-glycosylated), ~50 kDa (glycosylated pro form), ~43 kDa (predominant glycosylated soluble form) as well as other, smaller, soluble and membrane bound forms.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain tissue with Amphiregulin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain tissue with Amphiregulin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed rat PC-12 cells with Amphiregulin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Amphiregulin antibody.
