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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- Ab088632 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Amphiregulin Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Amphiregulin Antibody, Monoclonal(A3), could be used for WB, IHC, ICC/IF and so on . Application: WB: 0.5-3 µg/mL IHC: 5-30 µg/mL ICC/IF: 5-20 µg/mL Protein Function Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 20-100- Antibody clone number
- A3
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Amphiregulin Mouse mAb (Ab088632) - Western Blot All lanes: Amphiregulin Mouse mAb (Ab088632) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 29 kDa Exposure time: 38.2 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Amphiregulin Mouse mAb (Ab088632) - IF/ICC FITC staining on IF Sample: Human MCF7 cells Primary Ab: 20 μg/mL Amphiregulin Mouse mAb (Ab088632) Second Ab: 0.75 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Amphiregulin Mouse mAb (Ab088632) - IF/ICC FITC staining on IF Sample: MCF7 cells Primary Ab: 20 µg/mL Amphiregulin Mouse mAb (Ab088632) Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Amphiregulin Mouse mAb (Ab088632) - IHC DAB staining on IHC Sample: Human Breast cancer Tissue Primary Ab: 30 µg/mL Amphiregulin Mouse mAb (Ab088632) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
