PA5-27298

antibody from Invitrogen Antibodies

Targeting: AREG AREGB, SDGF

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27298

Provider

Invitrogen Antibodies

Product name

Amphiregulin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: AREG-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.17 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

In mouse chronic pancreatitis CD25(+)FOXP3(+) regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response.

Glaubitz J, Wilden A, Golchert J, Homuth G, Völker U, Bröker BM, Thiele T, Lerch MM, Mayerle J, Aghdassi AA, Weiss FU, Sendler M
Nature communications 2022 Aug 3;13(1):4502

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Amphiregulin activates human hepatic stellate cells and is upregulated in non alcoholic steatohepatitis.

McKee C, Sigala B, Soeda J, Mouralidarane A, Morgan M, Mazzoccoli G, Rappa F, Cappello F, Cabibi D, Pazienza V, Selden C, Roskams T, Vinciguerra M, Oben JA
Scientific reports 2015 Mar 6;5:8812

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 AR mRNA expression in hHSC. (A) Cultures of hHSC show significantly higher expression (* p < 0.05) of AR by day 4 compared to day 0. Expression is effectively reduced by day 10 (** p < 0.01). (B) AR mRNA expression is preceded by the up-regulation of the TACE gene, required for AR activity. TACE expression is highest at day 2 (*p < 0.05) and is reduced by day 4 (**p < 0.01). (C) Western blot analysis for AR and TACE protein levels in hHSC at day 0, 2, 4 and 10 (left panel); images were obtained from separate gels, run under the same experimental conditions, blotted on separate PVDF membranes and processed for detection with the respective antibodies. Densitometric quantification normalized to beta-actin levels (right panel). Images are representative of three independent experiments (* p < 0.05; ** p < 0.01). (D) TACE activities in hHSC at day 0, 2, 4 and 10 were measured using SensoLyte 520 TACE Activity Assay kit. N = 3, ** p < 0.01.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Effect of AR on collagen 1a2 production. (A) AR is capable of inducing collagen production at both picogram and nanogram concentrations (*p < 0.05). The effect is similar to that of TGF-beta, inductor of collagen production, a major step in the pathogenesis of fibrosis. (B) Mice were fed either a normal chow diet or a methionine-choline deficient (MCD) diet modelling NASH. Mice on MCD diet expressed higher hepatic AR mRNA levels than mice fed a normal chow diet (*p < 0.001). (C) Western blot analysis for AR protein levels in hepatocytes and mHSC isolated from MCD mice (left panel). Images were obtained from separate gels, run under the same experimental conditions, blotted on separate PVDF membranes and processed for detection with the respective antibodies. Densitometric quantification normalized to beta-actin levels (right panel). Images are representative of three independent experiments (* p < 0.05).