Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- ALX-210-949-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-210-949-C100, RRID:AB_2052163
- Product name
- Pannexin-1 (human) polyclonal antibody (AT124)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Epitope-affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot detection of human Pannexin-1 by PAb to Pannexin-1 (human) (AT124) (Prod. No ALX-210-949). Method: Cell extracts of HEK 293T mock-transfected (lane 1) or transfected with an expression vector encoding for human Pannexin-1 (lane 2) were run on a SDS-PAGE gel and analyzed by Western blot. After transfer, the nitrocellulose membrane was probed with PAb to Pannexin-1 (human) (AT124) at 1:1'000 for 1 hour at room temperature, and then incubated with an anti-rabbit horseradish peroxydase (1:4'000).