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Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- A303-830A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMD12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human PSMD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PSMD12 antibody A303-830A used for IP at 6 µg/mg lysate. PSMD12 was less efficiently immunoprecipitated by rabbit anti-PSMD12 antibody A303-829A, which recognizes an upstream epitope. For blotting immunoprecipitated PSMD12, A303-829A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
