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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-15735 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ataxin 1 Monoclonal Antibody (2F5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15735 targets ATXN1 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 2F5
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATXN1 using ATXN1 monoclonal antibody (Product # MA5-15735) in HEK293 (1) and ATXN1 (AA: 645-815) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATXN1 using ATXN1 monoclonal antibody (Product # MA5-15735) in HEK293 (1) and ATXN1 (AA: 645-815) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Ataxin 1 Monoclonal Antibody (2F5) (Product # MA5-15735) and a 95kDa band corresponding to Ataxin 1 was observed in SH-SY5Y and not in HEK-293 and Raji which are reported to be negative. Nuclear enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), HEK-293 (Lane 2) and Raji (Lane 3)were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NTERA-2 cells using ATXN1 monoclonal antibody (Product # MA5-15735) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and lung cancer tissues (right) using ATXN1 monoclonal antibody (Product # MA5-15735) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using ATXN1 monoclonal antibody (Product # MA5-15735) (green) and negative control (purple).
