Antibody data
- Antibody Data
- Antigen structure
- References [40]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 14139-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14139-1-AP, RRID:AB_2289230
- Product name
- ADAM12 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ADAM12 antibody (Cat. #14139-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references ADAM9 promotes type I interferon-mediated innate immunity during encephalomyocarditis virus infection.
ADAM12 promotes gemcitabine resistance by activating EGFR signaling pathway and induces EMT in bladder cancer.
ADAM12 promotes clear cell renal cell carcinoma progression and triggers EMT via EGFR/ERK signaling pathway.
Dysregulation of interaction between LOX(high) fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.
Vaccination-based immunotherapy to target profibrotic cells in liver and lung.
Impact of disintegrin and metalloproteinase domain-containing protein 12 on pancreatic ductal adenocarcinoma treated with surgical resection and perioperative chemotherapy.
Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations.
MicroRNA-149 Regulates Proliferation, Migration, and Invasion of Pituitary Adenoma Cells by Targeting ADAM12 and MMP14.
Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction.
Multi-organ metastasis as destination for breast cancer cells guided by biomechanical architecture.
Methylation status of ADAM12 promoter are associated with its expression levels in colorectal cancer.
Molecular characteristics of established trophoblast-derived cell lines.
Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT-20 and TtT/GF by affecting expression of MMP-14 and ADAM12.
ADAM12 silencing promotes cellular apoptosis by activating autophagy in choriocarcinoma cells.
Involvement of ADAM12 in Chondrocyte Differentiation by Regulation of TGF-β1-Induced IGF-1 and RUNX-2 Expressions.
SILAC Quantitative Proteomics and Biochemical Analyses Reveal a Novel Molecular Mechanism by Which ADAM12S Promotes the Proliferation, Migration, and Invasion of Small Cell Lung Cancer Cells through Upregulating Hexokinase 1.
ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.
Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway.
Genetic screens reveal mechanisms for the transcriptional regulation of tissue-specific genes in normal cells and tumors.
The transcriptional profiles and functional implications of long non-coding RNAs in the unfolded protein response.
Quantitative proteomics and biochemical analyses reveal the role of endoplasmin in the regulation of the expression and secretion of A Disintegrin And Metalloproteinase 12.
ADAM12 induces EMT and promotes cell migration, invasion and proliferation in pituitary adenomas via EGFR/ERK signaling pathway.
ADAM12 Is a Novel Regulator of Tumor Angiogenesis via STAT3 Signaling.
Sox10(+) adult stem cells contribute to biomaterial encapsulation and microvascularization.
Soluble Delta-like ligand 1 alters human endometrial epithelial cell adhesive capacity.
Metalloproteinases ADAM12 and MMP-14 are associated with cavernous sinus invasion in pituitary adenomas.
Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.
Erbb2 up-regulation of ADAM12 expression accelerates skin cancer progression.
Urinary ADAM12 and MMP-9/NGAL complex detect the presence of gastric cancer.
ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion.
Extravillous trophoblast-associated ADAM12 exerts pro-invasive properties, including induction of integrin beta 1-mediated cellular spreading.
A disintegrin and metalloproteinase 12 (ADAM12) localizes to invasive trophoblast, promotes cell invasion and directs column outgrowth in early placental development.
Notch-dependent RBPJκ inhibits proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts.
ADAM12 is expressed in the tumour vasculature and mediates ectodomain shedding of several membrane-anchored endothelial proteins.
A Disintegrin And Metalloproteinase 12 produced by tumour cells accelerates osteosarcoma tumour progression and associated osteolysis.
Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
Functional analysis of a breast cancer-associated mutation in the intracellular domain of the metalloprotease ADAM12.
A positive feedback loop between HER2 and ADAM12 in human head and neck cancer cells increases migration and invasion.
ADAM12 produced by tumor cells rather than stromal cells accelerates breast tumor progression.
ADAM12 transmembrane and secreted isoforms promote breast tumor growth: a distinct role for ADAM12-S protein in tumor metastasis.
Bazzone LE, Zhu J, King M, Liu G, Guo Z, MacKay CR, Kyawe PP, Qaisar N, Rojas-Quintero J, Owen CA, Brass AL, McDougall W, Baer CE, Cashman T, Trivedi CM, Gack MU, Finberg RW, Kurt-Jones EA
Nature communications 2024 May 16;15(1):4153
Nature communications 2024 May 16;15(1):4153
ADAM12 promotes gemcitabine resistance by activating EGFR signaling pathway and induces EMT in bladder cancer.
Wang R, Gao D, Chen C, Fan G, Cheng H, Tao Y, Lu J, Tian J, Wang Y, Wang Z
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2023 May;25(5):1425-1435
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2023 May;25(5):1425-1435
ADAM12 promotes clear cell renal cell carcinoma progression and triggers EMT via EGFR/ERK signaling pathway.
Xu J, Wang Y, Jiang J, Yin C, Shi B
Journal of translational medicine 2023 Jan 30;21(1):56
Journal of translational medicine 2023 Jan 30;21(1):56
Dysregulation of interaction between LOX(high) fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.
Chen Y, Zhang T, Yao F, Gao X, Li D, Fu S, Mao L, Liu F, Zhang X, Xu Y, Deng J, Li W, Fan G, Xiao C, Chen Y, Wang L, Guo W, Zhou B
Theranostics 2022;12(2):910-928
Theranostics 2022;12(2):910-928
Vaccination-based immunotherapy to target profibrotic cells in liver and lung.
Sobecki M, Chen J, Krzywinska E, Nagarajan S, Fan Z, Nelius E, Monné Rodriguez JM, Seehusen F, Hussein A, Moschini G, Hajam EY, Kiran R, Gotthardt D, Debbache J, Badoual C, Sato T, Isagawa T, Takeda N, Tanchot C, Tartour E, Weber A, Werner S, Loffing J, Sommer L, Sexl V, Münz C, Feghali-Bostwick C, Pachera E, Distler O, Snedeker J, Jamora C, Stockmann C
Cell stem cell 2022 Oct 6;29(10):1459-1474.e9
Cell stem cell 2022 Oct 6;29(10):1459-1474.e9
Impact of disintegrin and metalloproteinase domain-containing protein 12 on pancreatic ductal adenocarcinoma treated with surgical resection and perioperative chemotherapy.
Sakoda T, Uemura K, Kondo N, Sumiyoshi T, Okada K, Seo S, Otsuka H, Murakami Y, Takahashi S
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2022 May;22(4):479-487
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2022 May;22(4):479-487
Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations.
Leinroth AP, Mirando AJ, Rouse D, Kobayahsi Y, Tata PR, Rueckert HE, Liao Y, Long JT, Chakkalakal JV, Hilton MJ
Cell reports 2022 May 10;39(6):110785
Cell reports 2022 May 10;39(6):110785
MicroRNA-149 Regulates Proliferation, Migration, and Invasion of Pituitary Adenoma Cells by Targeting ADAM12 and MMP14.
Zhang Z, Schäfer A, Voellger B, Wang JW, Lei T, Nimsky C, Bartsch JW
Current medical science 2022 Dec;42(6):1131-1139
Current medical science 2022 Dec;42(6):1131-1139
Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction.
Klapproth E, Witt A, Klose P, Wiedemann J, Vavilthota N, Künzel SR, Kämmerer S, Günscht M, Sprott D, Lesche M, Rost F, Dahl A, Rauch E, Kattner L, Weber S, Mirtschink P, Kopaliani I, Guan K, Lorenz K, Saftig P, Wagner M, El-Armouche A
Nature communications 2022 Dec 10;13(1):7648
Nature communications 2022 Dec 10;13(1):7648
Multi-organ metastasis as destination for breast cancer cells guided by biomechanical architecture.
Lin Q, Chen X, Meng F, Ogawa K, Li M, Song R, Zhang S, Zhang Z, Kong X, Xu Q, He F, Liu D, Bai X, Sun B, Hung MC, Liu L, Wands JR, Dong X
American journal of cancer research 2021;11(6):2537-2567
American journal of cancer research 2021;11(6):2537-2567
Methylation status of ADAM12 promoter are associated with its expression levels in colorectal cancer.
Wang Y, Zhang J, Cao H, Han F, Zhang H, Xu E
Pathology, research and practice 2021 May;221:153449
Pathology, research and practice 2021 May;221:153449
Molecular characteristics of established trophoblast-derived cell lines.
Pastuschek J, Nonn O, Gutiérrez-Samudio RN, Murrieta-Coxca JM, Müller J, Sanft J, Huppertz B, Markert UR, Groten T, Morales-Prieto DM
Placenta 2021 May;108:122-133
Placenta 2021 May;108:122-133
Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT-20 and TtT/GF by affecting expression of MMP-14 and ADAM12.
Zhang Z, Bartsch JW, Benzel J, Lei T, Nimsky C, Voellger B
FEBS open bio 2020 Nov;10(11):2489-2498
FEBS open bio 2020 Nov;10(11):2489-2498
ADAM12 silencing promotes cellular apoptosis by activating autophagy in choriocarcinoma cells.
Wang L, Tan Z, Zhang Y, Kady Keita N, Liu H, Zhang Y
International journal of oncology 2020 May;56(5):1162-1174
International journal of oncology 2020 May;56(5):1162-1174
Involvement of ADAM12 in Chondrocyte Differentiation by Regulation of TGF-β1-Induced IGF-1 and RUNX-2 Expressions.
Horita M, Nishida K, Hasei J, Furumatsu T, Sakurai M, Onodera Y, Fukuda K, Salter DM, Ozaki T
Calcified tissue international 2019 Jul;105(1):97-106
Calcified tissue international 2019 Jul;105(1):97-106
SILAC Quantitative Proteomics and Biochemical Analyses Reveal a Novel Molecular Mechanism by Which ADAM12S Promotes the Proliferation, Migration, and Invasion of Small Cell Lung Cancer Cells through Upregulating Hexokinase 1.
Duan Q, Li D, Xiong L, Chang Z, Xu G
Journal of proteome research 2019 Jul 5;18(7):2903-2914
Journal of proteome research 2019 Jul 5;18(7):2903-2914
ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.
Li W, Wang D, Sun X, Zhang Y, Wang L, Suo J
International journal of molecular medicine 2019 Feb;43(2):914-926
International journal of molecular medicine 2019 Feb;43(2):914-926
Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway.
Ogawa K, Lin Q, Li L, Bai X, Chen X, Chen H, Kong R, Wang Y, Zhu H, He F, Xu Q, Liu L, Li M, Zhang S, Nagaoka K, Carlson R, Safran H, Charpentier K, Sun B, Wands J, Dong X
Journal of hematology & oncology 2019 Dec 30;12(1):144
Journal of hematology & oncology 2019 Dec 30;12(1):144
Genetic screens reveal mechanisms for the transcriptional regulation of tissue-specific genes in normal cells and tumors.
Naciri I, Laisné M, Ferry L, Bourmaud M, Gupta N, Di Carlo S, Huna A, Martin N, Peduto L, Bernard D, Kirsh O, Defossez PA
Nucleic acids research 2019 Apr 23;47(7):3407-3421
Nucleic acids research 2019 Apr 23;47(7):3407-3421
The transcriptional profiles and functional implications of long non-coding RNAs in the unfolded protein response.
Quan H, Fan Q, Li C, Wang YY, Wang L
Scientific reports 2018 Mar 21;8(1):4981
Scientific reports 2018 Mar 21;8(1):4981
Quantitative proteomics and biochemical analyses reveal the role of endoplasmin in the regulation of the expression and secretion of A Disintegrin And Metalloproteinase 12.
Xiong L, Yan W, Zubia E, Zhou Y, Zhang Y, Duan Q, Narayan M, Xu G
Journal of proteomics 2018 Jun 30;182:34-44
Journal of proteomics 2018 Jun 30;182:34-44
ADAM12 induces EMT and promotes cell migration, invasion and proliferation in pituitary adenomas via EGFR/ERK signaling pathway.
Wang J, Zhang Z, Li R, Mao F, Sun W, Chen J, Zhang H, Bartsch JW, Shu K, Lei T
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Jan;97:1066-1077
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2018 Jan;97:1066-1077
ADAM12 Is a Novel Regulator of Tumor Angiogenesis via STAT3 Signaling.
Roy R, Dagher A, Butterfield C, Moses MA
Molecular cancer research : MCR 2017 Nov;15(11):1608-1622
Molecular cancer research : MCR 2017 Nov;15(11):1608-1622
Sox10(+) adult stem cells contribute to biomaterial encapsulation and microvascularization.
Wang D, Wang A, Wu F, Qiu X, Li Y, Chu J, Huang WC, Xu K, Gong X, Li S
Scientific reports 2017 Jan 10;7:40295
Scientific reports 2017 Jan 10;7:40295
Soluble Delta-like ligand 1 alters human endometrial epithelial cell adhesive capacity.
Van Sinderen M, Oyanedel J, Menkhorst E, Cuman C, Rainczuk K, Winship A, Salamonsen L, Edgell T, Dimitriadis E
Reproduction, fertility, and development 2017 Apr;29(4):694-702
Reproduction, fertility, and development 2017 Apr;29(4):694-702
Metalloproteinases ADAM12 and MMP-14 are associated with cavernous sinus invasion in pituitary adenomas.
Wang J, Voellger B, Benzel J, Schlomann U, Nimsky C, Bartsch JW, Carl B
International journal of cancer 2016 Sep 15;139(6):1327-39
International journal of cancer 2016 Sep 15;139(6):1327-39
Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.
Zhou Y, Xiong L, Zhang Y, Yu R, Jiang X, Xu G
Journal of proteomics 2016 Oct 4;148:94-104
Journal of proteomics 2016 Oct 4;148:94-104
Erbb2 up-regulation of ADAM12 expression accelerates skin cancer progression.
Rao VH, Vogel K, Yanagida JK, Marwaha N, Kandel A, Trempus C, Repertinger SK, Hansen LA
Molecular carcinogenesis 2015 Oct;54(10):1026-36
Molecular carcinogenesis 2015 Oct;54(10):1026-36
Urinary ADAM12 and MMP-9/NGAL complex detect the presence of gastric cancer.
Shimura T, Dagher A, Sachdev M, Ebi M, Yamada T, Yamada T, Joh T, Moses MA
Cancer prevention research (Philadelphia, Pa.) 2015 Mar;8(3):240-8
Cancer prevention research (Philadelphia, Pa.) 2015 Mar;8(3):240-8
ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion.
Aghababaei M, Hogg K, Perdu S, Robinson WP, Beristain AG
Cell death and differentiation 2015 Dec;22(12):1970-84
Cell death and differentiation 2015 Dec;22(12):1970-84
Extravillous trophoblast-associated ADAM12 exerts pro-invasive properties, including induction of integrin beta 1-mediated cellular spreading.
Biadasiewicz K, Fock V, Dekan S, Proestling K, Velicky P, Haider S, Knöfler M, Fröhlich C, Pollheimer J
Biology of reproduction 2014 May;90(5):101
Biology of reproduction 2014 May;90(5):101
A disintegrin and metalloproteinase 12 (ADAM12) localizes to invasive trophoblast, promotes cell invasion and directs column outgrowth in early placental development.
Aghababaei M, Perdu S, Irvine K, Beristain AG
Molecular human reproduction 2014 Mar;20(3):235-49
Molecular human reproduction 2014 Mar;20(3):235-49
Notch-dependent RBPJκ inhibits proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts.
Velicky P, Haider S, Otti GR, Fiala C, Pollheimer J, Knöfler M
Molecular human reproduction 2014 Aug;20(8):756-66
Molecular human reproduction 2014 Aug;20(8):756-66
ADAM12 is expressed in the tumour vasculature and mediates ectodomain shedding of several membrane-anchored endothelial proteins.
Fröhlich C, Klitgaard M, Noer JB, Kotzsch A, Nehammer C, Kronqvist P, Berthelsen J, Blobel C, Kveiborg M, Albrechtsen R, Wewer UM
The Biochemical journal 2013 May 15;452(1):97-109
The Biochemical journal 2013 May 15;452(1):97-109
A Disintegrin And Metalloproteinase 12 produced by tumour cells accelerates osteosarcoma tumour progression and associated osteolysis.
Georges S, Chesneau J, Hervouet S, Taurelle J, Gouin F, Redini F, Padrines M, Heymann D, Fortun Y, Verrecchia F
European journal of cancer (Oxford, England : 1990) 2013 Jun;49(9):2253-63
European journal of cancer (Oxford, England : 1990) 2013 Jun;49(9):2253-63
Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
Díaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge SA
The Journal of cell biology 2013 Apr 15;201(2):279-92
The Journal of cell biology 2013 Apr 15;201(2):279-92
Functional analysis of a breast cancer-associated mutation in the intracellular domain of the metalloprotease ADAM12.
Stautz D, Wewer UM, Kveiborg M
PloS one 2012;7(5):e37628
PloS one 2012;7(5):e37628
A positive feedback loop between HER2 and ADAM12 in human head and neck cancer cells increases migration and invasion.
Rao VH, Kandel A, Lynch D, Pena Z, Marwaha N, Deng C, Watson P, Hansen LA
Oncogene 2012 Jun 7;31(23):2888-98
Oncogene 2012 Jun 7;31(23):2888-98
ADAM12 produced by tumor cells rather than stromal cells accelerates breast tumor progression.
Fröhlich C, Nehammer C, Albrechtsen R, Kronqvist P, Kveiborg M, Sehara-Fujisawa A, Mercurio AM, Wewer UM
Molecular cancer research : MCR 2011 Nov;9(11):1449-61
Molecular cancer research : MCR 2011 Nov;9(11):1449-61
ADAM12 transmembrane and secreted isoforms promote breast tumor growth: a distinct role for ADAM12-S protein in tumor metastasis.
Roy R, Rodig S, Bielenberg D, Zurakowski D, Moses MA
The Journal of biological chemistry 2011 Jun 10;286(23):20758-68
The Journal of biological chemistry 2011 Jun 10;286(23):20758-68
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP(ADAM12 antibody) at dilution of 1:300
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using ADAM12 antibody 14139-1-AP at 1:25 and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human endometrial cancer using 14139-1-AP(ADAM12 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human endometrial cancer using 14139-1-AP(ADAM12 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue