644201

antibody from BioLegend

Targeting: SKIL SNO, SnoA, SnoN

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

644201

Provider

BioLegend

Proper citation

BioLegend Cat#644201, RRID:AB_2715795

Product name

Purified anti-SKIL (SnoN)

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

O91H2

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from HEK293T (negative control, lane 1), NTERA-2 (lane 2), and HaCaT (lane 3) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) purified anti-SKIL (SnoN) antibody (clone O91H2, left) antibody and a competitor’s antibody used at the manufacturer’s recommended concentration (right). Proteins from each membrane were visualized by chemiluminescence detection using goat anti-mouse-IgG secondary antibody conjugated to HRP at a 1:3000 dilution. Direct-Blot™ β-Actin was used as a loading control at a 1:2000 dilution (lower). Lane M: Molecular weight ladder.

Conjugate

Unconjugated