Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 644202 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#644202, RRID:AB_2715796
- Product name
- Purified anti-SKIL (SnoN)
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- O91H2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Total cell lysates (15 µg protein) from HEK293T (negative control, lane 1), NTERA-2 (lane 2), and HaCaT (lane 3) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) purified anti-SKIL (SnoN) antibody (clone O91H2, left) antibody and a competitor’s antibody used at the manufacturer’s recommended concentration (right). Proteins from each membrane were visualized by chemiluminescence detection using goat anti-mouse-IgG secondary antibody conjugated to HRP at a 1:3000 dilution. Direct-Blot™ β-Actin was used as a loading control at a 1:2000 dilution (lower). Lane M: Molecular weight ladder.
- Conjugate
- Unconjugated