10705-1-AP

antibody from Proteintech Group

Targeting: NELFE D6S45, NELF-E, RD, RDBP, RDP

Provider product page for 10705-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [23]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 10705-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10705-1-AP, RRID:AB_513966
Product name: NELFE antibody
Antibody type: Polyclonal
Description: KD/KO validated NELFE antibody (Cat. #10705-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NELFE protein structure - 10705-1-AP shown in red.

Submitted references Highly Expressed NELFE in Tumor Cells at the Invasive Tumor Front Was a Prognostic Biomarker for Oral Squamous Cell Carcinoma.

Xue Y, Liu L, Yu L, Li Z, Huang X, Chen S, Jing Y, Zhang X, Ding L, Song Y, Wang Z, Ni Y
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2025 Sep;54(8):757-769

Nelfb promotes dermal white adipose tissue formation through RNA polymerase II-mediated adipogenic gene regulation.

Mahapatra S, Gomez J, Batzorig U, Liu Y, Fernández-Méndez C, Chen Y, Sen GL
Development (Cambridge, England) 2025 Oct 15;152(20)

Gen-17, a beta-methyl derivative of Genipin, attenuates LPS-induced ALI by regulating Keap1-Nrf2/HO-1 and suppressing NF-κB and MAPK-dependent signaling pathways.

Zhu YS, Shah SAA, Yang BY, Fan SS, He L, Sun YR, Shang WB, Qian Y, Zhang X
Biochimica et biophysica acta. Molecular basis of disease 2025 Jun;1871(5):167770

Chemical catalyst manipulating cancer epigenome and transcription.

Yamanashi Y, Takamaru S, Okabe A, Kaito S, Azumaya Y, Kamimura YR, Yamatsugu K, Kujirai T, Kurumizaka H, Iwama A, Kaneda A, Kawashima SA, Kanai M
Nature communications 2025 Jan 24;16(1):887

Mutual regulation between cell cycle and transcription termination factor TTF2.

Hu Y, Xu L, Feng B, Wu Q, Wang Y, Gong W, Lv M, Zhao H, Chen J, Li J, Zhang W, Zhan Q
Science China. Life sciences 2025 Aug;68(8):2348-2362

DOT1L/H3K79me2 represses HIV-1 reactivation via recruiting DCAF1.

Liang F, Jin J, Li Q, Duan J, Jiang A, Chen X, Geng H, Wu K, Yu F, Zhao X, Zhou Y, Hu D, Chen L
Cell reports 2024 Jul 23;43(7):114368

An essential signaling function of cytoplasmic NELFB is independent of RNA polymerase II pausing.

Pan H, Cheng X, Rodríguez PFG, Zhang X, Chung I, Jin VX, Li W, Hu Y, Li R
The Journal of biological chemistry 2023 Nov;299(11):105259

Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve.

Chignon A, Argaud D, Boulanger MC, Mkannez G, Bon-Baret V, Li Z, Thériault S, Bossé Y, Mathieu P
PLoS genetics 2022 Jan;18(1):e1010010

Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.

Ou J, Guan X, Wang J, Wang T, Zhang B, Li R, Xu H, Hu X, Guo XK
Mucosal immunology 2022 Feb;15(2):279-288

RNA polymerase II pausing factor NELF in CD8(+) T cells promotes antitumor immunity.

Wu B, Zhang X, Chiang HC, Pan H, Yuan B, Mitra P, Qi L, Simonyan H, Young CN, Yvon E, Hu Y, Zhang N, Li R
Nature communications 2022 Apr 20;13(1):2155

The RNA-Binding Protein NELFE Promotes Gastric Cancer Growth and Metastasis Through E2F2.

Chen C, Zheng Q, Pan S, Chen W, Huang J, Cao Y, Tu Y, Li Z, Yu C, Jie Z
Frontiers in oncology 2021;11:677111

Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag.

Li Y, Nakka K, Olender T, Gingras-Gelinas P, Wong MM, Robinson DCL, Bandukwala H, Palii CG, Neyret O, Brand M, Blais A, Dilworth FJ
STAR protocols 2021 Sep 17;2(3):100751

Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.

Robinson DCL, Ritso M, Nelson GM, Mokhtari Z, Nakka K, Bandukwala H, Goldman SR, Park PJ, Mounier R, Chazaud B, Brand M, Rudnicki MA, Adelman K, Dilworth FJ
Developmental cell 2021 Apr 5;56(7):1014-1029.e7

The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.

Liu B, Xu Q, Wang Q, Feng S, Lai F, Wang P, Zheng F, Xiang Y, Wu J, Nie J, Qiu C, Xia W, Li L, Yu G, Lin Z, Xu K, Xiong Z, Kong F, Liu L, Huang C, Yu Y, Na J, Xie W
Nature 2020 Nov;587(7832):139-144

Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.

Yu L, Zhang B, Deochand D, Sacta MA, Coppo M, Shang Y, Guo Z, Zeng X, Rollins DA, Tharmalingam B, Li R, Chinenov Y, Rogatsky I, Hu X
Nature communications 2020 May 8;11(1):2286

Negative elongation factor is essential for endometrial function.

Hewitt SC, Li R, Adams N, Winuthayanon W, Hamilton KJ, Donoghue LJ, Lierz SL, Garcia M, Lydon JP, DeMayo FJ, Adelman K, Korach KS
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb;33(2):3010-3023

Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages.

Sacta MA, Tharmalingam B, Coppo M, Rollins DA, Deochand DK, Benjamin B, Yu L, Zhang B, Hu X, Li R, Chinenov Y, Rogatsky I
eLife 2018 Feb 9;7

Striatal NELF-mediated RNA polymerase II stalling controls L-dopa induced dyskinesia.

Bastide MF, Bido S, Duteil N, Bézard E
Neurobiology of disease 2016 Jan;85:93-98

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Lu X, Zhu X, Li Y, Liu M, Yu B, Wang Y, Rao M, Yang H, Zhou K, Wang Y, Chen Y, Chen M, Zhuang S, Chen LF, Liu R, Chen R
Nucleic acids research 2016 Aug 19;44(14):6853-67

Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle.

Gupte R, Muse GW, Chinenov Y, Adelman K, Rogatsky I
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 3;110(36):14616-21

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Sawarkar R, Sievers C, Paro R
Cell 2012 May 11;149(4):807-18

Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma.

Iida M, Iizuka N, Tsunedomi R, Tsutsui M, Yoshida S, Maeda Y, Tokuhisa Y, Sakamoto K, Yoshimura K, Tamesa T, Oka M
Oncology reports 2012 Aug;28(2):728-34

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Adelman K, Kennedy MA, Nechaev S, Gilchrist DA, Muse GW, Chinenov Y, Rogatsky I
Proceedings of the National Academy of Sciences of the United States of America 2009 Oct 27;106(43):18207-12

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10705-1-AP(RDBP antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The NELFE antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NELFE. This antibody recognizes human,mouse,rat antigen. The NELFE antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 10705-1-AP.pdf

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