HPA007187

D6S45, NELF-E, RD, RDBP, RDP

Eligible for validation within the Antibodypedia Validation Initiative

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Validation data

Product number: HPA007187
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA007187, RRID:AB_1235415
Product name: Anti-NELFE
Provider product page: Atlas Antibodies - HPA007187
Antibody type: Polyclonal
Description: Affinity purified using the PrEST antigen as affinity ligand
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: LLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATE QAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKD PEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLY ESFVSSSDRLRELGPDG
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human small intestine shows weak to moderate nuclear positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA007187 antibody. Corresponding NELFE RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.