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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB099 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Leukotriene B4 R1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Recognizes human Leukotriene B4 R1 (Pettersson, A. et al. (2000) Biochem. Biophys. Res. Commun. 279:520).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Low leukotriene B4 receptor 1 leads to ALOX5 downregulation at diagnosis of chronic myeloid leukemia.
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Lucas CM, Harris RJ, Giannoudis A, McDonald E, Clark RE
Haematologica 2014 Nov;99(11):1710-5
Haematologica 2014 Nov;99(11):1710-5
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Cabezón R, Sintes J, Llinàs L, Benitez-Ribas D
Immunology letters 2011 Jan 30;134(2):167-73
Immunology letters 2011 Jan 30;134(2):167-73
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monoctyes were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 Monoclonal Antibody (Catalog # MAB099) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
