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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- 700902 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSD-95 Recombinant Rabbit Monoclonal Antibody (23H23L19)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 700902 has been successfully used in immunofluorescence analysis of PSD95 in human motor neurons derived from iPSCs.
- Antibody clone number
- 23H23L19
- Concentration
- 0.5 mg/mL
Submitted references Synaptamide Improves Cognitive Functions and Neuronal Plasticity in Neuropathic Pain.
Small molecule inhibitors of PSD95-nNOS protein-protein interactions suppress formalin-evoked Fos protein expression and nociceptive behavior in rats.
Tyrtyshnaia A, Bondar A, Konovalova S, Manzhulo I
International journal of molecular sciences 2021 Nov 26;22(23)
International journal of molecular sciences 2021 Nov 26;22(23)
Small molecule inhibitors of PSD95-nNOS protein-protein interactions suppress formalin-evoked Fos protein expression and nociceptive behavior in rats.
Carey LM, Lee WH, Gutierrez T, Kulkarni PM, Thakur GA, Lai YY, Hohmann AG
Neuroscience 2017 May 4;349:303-317
Neuroscience 2017 May 4;349:303-317
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSD-95 in rat brain (4-weeks) cell lysate (30 µg/lane) using a PSD-95 recombinant rabbit monoclonal antibody (Product # 700902) at a dilution of 0.1 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PSD-95 Recombinant Rabbit Monoclonal Antibody (23H23L19) (Product # 700902) and a 80 kDa band corresponding to PSD-95 was observed across U-87 MG and SH-SY5Y. Whole Cell Extract-WCL (30 µg lysate) of U-87 MG (Lane 1), SH-SY5Y (Lane 2), Hep G2 (Lane 3), HeLa (Lane 4) and SiHa (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PSD95 (red) in human motor neurons derived from iPSCs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.2% Triton X-100 in PBS for 10 minutes, and blocked with 5% donkey serum in PBS for 15 minutes at room temperature. Cells were stained with a PSD95 rabbit monoclonal antibody (Product # 700902) diluted at 1:1000 in 5% donkey serum overnight at 4°C, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 546 conjugate (Product # A-11010) at a dilution of 1:1000 for 1 hour at room temperature (green). Note: Data courtesy of Innovators Program.
