51-6900

antibody from Invitrogen Antibodies

Targeting: DLG4 PSD-95, PSD95, SAP-90, SAP90

Antibody data

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Validation data

Product number: 51-6900 - Provider product page
Provider: Invitrogen Antibodies
Product name: PSD-95 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody recognizes the 95 kDa PSD-95 protein. Cross-reactivity with an unknown protein at ~50 kDa has been observed in western blots with rat brain homogenates. Western blots with mouse brain homogenates did not show such a band.
Concentration: 0.25 mg/mL

DLG4 protein structure - 51-6900 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of postsynaptic density protein (PSD-95) in E18 Sparague Dawley primary cortical neuronal cells. The cells were fixed with 4% formaldehyde for 15 mins, permeabilized with 0.25% Triton X-100 in PBS for 10 mins, and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a PSD-95 rabbit polyclonal antibody (Product # 51-6900) at a dilution of 1:200 in 3% BSA in PBS for 1 hr at RT, and then incubated with Invitrogen AlexaFluor 647 Plus goat anti-rabbit IgG secondary antibody (Product # A32733) at a dilution of 1:1000 for 1 hr at RT. Nuclei were stained with Hoechst 33342 (Product # H3570). The image contains overlay of neuronal PSD-95 (red) and nuclei (blue). Images were taken on a Zeiss LSM 710 confocal microscope at 40X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PSD-95 (green) and MAP2 (red) on rat primary cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System (Product # A3653401). At day 28 the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% triton X-100 for 30min, and blocked with 1% BSA for 30 min at room temperature. Cells were stained with anti-PSD95 antibody (Product # 51-6900) at a dilution of 1:200, and anti-MAP2 (Product # 13-1500) at a dilution of 1:400, in 1% BSA staining buffer, overnight at 4C, and then incubated with Alexa Fluor 488 conjugated donkey anti-rabbit (Product # A-21206) and Alexa Fluor 594 donkey anti-mouse (Product # A-21203) antibodies at a dilution of 1:1000 for 30 min. at room temp. Wash 3 times with DPBS. Stain with DAPI for nucleus.