Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-48538 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC16A10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC16A10 in (Lane 1) human skeletal muscle lysates (20 µg), (Lane 2) CCRF-CEM whole cell lysates (20 µg). Samples probed with a SLC16A10 Antibody (C-term) (Product # PA5-48538) at a 1:2000 dilution, followed by Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC16A10 in K562 cell line lysates (35 µg/lane). Samples were probed with a SLC16A10 Antibody (C-term) (Product # PA5-48538).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SLC16A10 in paraformaldehyde-fixed, paraffin-embedded human skeletal muscle tissue. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with a SLC16A10 polyclonal Antibody (Product # PA5-48538) at a 1:25 dilution for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SLC16A10 in formalin fixed and paraffin embedded human skeletal muscle. Samples were probed with a SLC16A10 Antibody (C-term) (Product # PA5-48538) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SLC16A10 in K562 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a SLC16A10 Antibody (C-term) (Product # PA5-48538). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.