PA5-48538

antibody from Invitrogen Antibodies

Targeting: SLC16A10 MCT10, TAT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-48538

Provider

Invitrogen Antibodies

Product name

SLC16A10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC16A10 in various lysates. Samples were incubated with SLC16A10 polyclonal antibody (Product # PA5-48538) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: human skeletal muscle lysates; Lane 2: CCRF-CEM whole cell lysates. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC16A10 in K562 cell line lysates. Samples were incubated with SLC16A10 polyclonal antibody (Product # PA5-48538). Lysates: 35 µg/lane. SLC16A10 protein (arrow).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SLC16A10 in paraformaldehyde-fixed, paraffin-embedded human skeletal muscle tissue sections. Samples were incubated with SLC16A10 polyclonal antibody (Product # PA5-48538) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SLC16A10 in formalin fixed and paraffin embedded human skeletal muscle. Samples were incubated with SLC16A10 polyclonal antibody (Product # PA5-48538) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SLC16A10 in paraformaldehyde-fixed, paraffin-embedded human skeletal muscle tissue sections. Samples were incubated with SLC16A10 polyclonal antibody (Product # PA5-48538) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of SLC16A10 in K562 cells (right histogram) compared to a negative control cell (left histogram). Samples were probed with a SLC16A10 Antibody (C-term) (Product # PA5-48538). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SLC16A10 in K562 cells (right histogram). Samples were incubated with SLC16A10 polyclonal antibody (Product # PA5-48538) followed by FITC-conjugated donkey-anti-rabbit secondary antibody. Negative control (left histogram).