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antibody from Invitrogen Antibodies
Targeting: ADIPOQ
ACDC, ACRP30, adiponectin, apM1, GBP28
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-84881 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adiponectin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. In Western blot, this antibody detects a band at ~26.4kDa in human HL60 cell lysates. A suggested positive control for PA1-84881 is rat brain. Rabbit anti Human adiponectin antibody recognizes an epitope within the C-terminal region (CT) of the 244 amino acid protein adiponectin (also known as Acrp30), a major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Serum concentration and vascular expression of adiponectin are differentially associated with the diabetic calcifying peripheral arteriopathy.
Aubert CE, Liabeuf S, Amouyal C, Kemel S, Lajat-Kiss F, Lacorte JM, Halbron M, Carlier A, Salem JE, Funck-Brentano C, Perisic Matic L, Witasp A, Stenvinkel P, Phan F, Massy ZA, Hartemann A, Bourron O
Diabetology & metabolic syndrome 2019;11:32
Diabetology & metabolic syndrome 2019;11:32
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of whole cell lysate from HL60 promyelocytic leukemia cells probed with a Adiponectin polyclonal antibody (Product # PA1-84881) at 0.5 (A), 1 (B) and 2 (C) µg/mL
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical staining of HL60 cells using a Adiponectin polyclonal antibody (Product # PA1-84881).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of rat brain using a Adiponectin polyclonal antibody (Product # PA1-84881)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Immunohistochemical staining of adiponectin in arteries from patients with type 2 diabetes. Adiponectin (ADIPOQ) protein expression and localization was examined by immunohistochemistry staining in arteries from diabetic patients, classified according to the grade of calcification. ADIPOQ (red signal) was found to be expressed in all stages of calcification, but it was particularly enriched in early stages (grade 1). Smooth muscle cells of the media were specifically immuno positive for ADIPOQ, but the signal was also present in microvessels of the adventitia (particularly grade 2). Images were taken with x5 magnification and enlarged images with x15 (size bars included)
