MAB10652

antibody from Novus Biologicals

Targeting: ADIPOQ ACDC, ACRP30, adiponectin, apM1, GBP28

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB10652

Provider

Novus Biologicals

Product name

Mouse Monoclonal Adiponectin/Acrp30 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Adiponectin/Acrp30 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Acrp30 (aa 101-244), recombinant mouse (rm) Acrp30 (aa 111-247), rmAcrp30 (HWM), recombinant rat Acrp30 (aa 1-244), rhC1qTNF1, rmCORS26/C1qTNF3, rhC1qTNF4, rhC1qTNF5, rhC1qTNF9, or rhC1qL2 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Potential Roles of Adiponectin Isoforms in Human Obesity with Delayed Wound Healing.

Ryu J, Loza CA, Xu H, Zhou M, Hadley JT, Wu J, You H, Wang H, Yang J, Bai J, Liu F, Bialowas C, Dong LQ
Cells 2019 Sep 24;8(10)

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Functional effects of adiponectin on endothelial progenitor cells.

Lavoie V, Kernaleguen AE, Charron G, Farhat N, Cossette M, Mamarbachi AM, Allen BG, Rhéaume E, Tardif JC
Obesity (Silver Spring, Md.) 2011 Apr;19(4):722-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of human adipose tissue. PVDF Membrane was probed with 2 µg/mL of Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10652) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Adiponectin/Acrp30 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.