Antibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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- Product number
- AF1119 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Adiponectin/Acrp30 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant rat Adiponectin is observed and less than 30% cross-reactivity with recombinant human Adiponectin is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q60994
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Adiponectin modulates ventral tegmental area dopamine neuron activity and anxiety-related behavior through AdipoR1.
SDF-1 Is an Autocrine Insulin-Desensitizing Factor in Adipocytes.
Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice.
Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.
The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2.
Retinol-binding protein 7 is an endothelium-specific PPARγ cofactor mediating an antioxidant response through adiponectin.
Role of the adipose PPARγ-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors.
Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice.
Positive feedback regulation between adiponectin and T-cadherin impacts adiponectin levels in tissue and plasma of male mice.
Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice.
Adiponectin promotes monocyte-to-fibroblast transition in renal fibrosis.
Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
T-cadherin is essential for adiponectin-mediated revascularization.
Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype.
Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions.
Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies.
Sun F, Lei Y, You J, Li C, Sun L, Garza J, Zhang D, Guo M, Scherer PE, Lodge D, Lu XY
Molecular psychiatry 2019 Jan;24(1):126-144
Molecular psychiatry 2019 Jan;24(1):126-144
SDF-1 Is an Autocrine Insulin-Desensitizing Factor in Adipocytes.
Shin J, Fukuhara A, Onodera T, Kita S, Yokoyama C, Otsuki M, Shimomura I
Diabetes 2018 Jun;67(6):1068-1078
Diabetes 2018 Jun;67(6):1068-1078
Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice.
Quiroga DT, Muñoz MC, Gil C, Pffeifer M, Toblli JE, Steckelings UM, Giani JF, Dominici FP
Physiological reports 2018 Aug;6(16):e13824
Physiological reports 2018 Aug;6(16):e13824
Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.
Obata Y, Kita S, Koyama Y, Fukuda S, Takeda H, Takahashi M, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nakamura Y, Nishizawa H, Funahashi T, Ranscht B, Izumi Y, Bamba T, Fukusaki E, Hanayama R, Shimada S, Maeda N, Shimomura I
JCI insight 2018 Apr 19;3(8)
JCI insight 2018 Apr 19;3(8)
The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2.
Fukuda S, Kita S, Obata Y, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nishizawa H, Funahashi T, Takagi J, Maeda N, Shimomura I
The Journal of biological chemistry 2017 May 12;292(19):7840-7849
The Journal of biological chemistry 2017 May 12;292(19):7840-7849
Retinol-binding protein 7 is an endothelium-specific PPARγ cofactor mediating an antioxidant response through adiponectin.
Hu C, Keen HL, Lu KT, Liu X, Wu J, Davis DR, Ibeawuchi SC, Vogel S, Quelle FW, Sigmund CD
JCI insight 2017 Mar 23;2(6):e91738
JCI insight 2017 Mar 23;2(6):e91738
Role of the adipose PPARγ-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors.
Guo M, Li C, Lei Y, Xu S, Zhao D, Lu XY
Molecular psychiatry 2017 Jul;22(7):1056-1068
Molecular psychiatry 2017 Jul;22(7):1056-1068
Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice.
Muñoz MC, Burghi V, Miquet JG, Cervino IA, Quiroga DT, Mazziotta L, Dominici FP
Peptides 2017 Feb;88:37-45
Peptides 2017 Feb;88:37-45
Positive feedback regulation between adiponectin and T-cadherin impacts adiponectin levels in tissue and plasma of male mice.
Matsuda K, Fujishima Y, Maeda N, Mori T, Hirata A, Sekimoto R, Tsushima Y, Masuda S, Yamaoka M, Inoue K, Nishizawa H, Kita S, Ranscht B, Funahashi T, Shimomura I
Endocrinology 2015 Mar;156(3):934-46
Endocrinology 2015 Mar;156(3):934-46
Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice.
Mori T, Koyama Y, Maeda N, Nakamura Y, Fujishima Y, Matsuda K, Funahashi T, Shimada S, Shimomura I
Scientific reports 2014 May 8;4:4895
Scientific reports 2014 May 8;4:4895
Adiponectin promotes monocyte-to-fibroblast transition in renal fibrosis.
Yang J, Lin SC, Chen G, He L, Hu Z, Chan L, Trial J, Entman ML, Wang Y
Journal of the American Society of Nephrology : JASN 2013 Oct;24(10):1644-59
Journal of the American Society of Nephrology : JASN 2013 Oct;24(10):1644-59
Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
Kim HA, Kwon NS, Baek KJ, Kim DS, Yun HY
Cytokine 2013 May;62(2):206-9
Cytokine 2013 May;62(2):206-9
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Driskell RR, Lichtenberger BM, Hoste E, Kretzschmar K, Simons BD, Charalambous M, Ferron SR, Herault Y, Pavlovic G, Ferguson-Smith AC, Watt FM
Nature 2013 Dec 12;504(7479):277-281
Nature 2013 Dec 12;504(7479):277-281
T-cadherin is essential for adiponectin-mediated revascularization.
Parker-Duffen JL, Nakamura K, Silver M, Kikuchi R, Tigges U, Yoshida S, Denzel MS, Ranscht B, Walsh K
The Journal of biological chemistry 2013 Aug 23;288(34):24886-97
The Journal of biological chemistry 2013 Aug 23;288(34):24886-97
Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype.
Ding M, Carrão AC, Wagner RJ, Xie Y, Jin Y, Rzucidlo EM, Yu J, Li W, Tellides G, Hwa J, Aprahamian TR, Martin KA
Journal of molecular and cellular cardiology 2012 Feb;52(2):474-84
Journal of molecular and cellular cardiology 2012 Feb;52(2):474-84
Molecular, biochemical and functional characterizations of C1q/TNF family members: adipose-tissue-selective expression patterns, regulation by PPAR-gamma agonist, cysteine-mediated oligomerizations, combinatorial associations and metabolic functions.
Wong GW, Krawczyk SA, Kitidis-Mitrokostas C, Revett T, Gimeno R, Lodish HF
The Biochemical journal 2008 Dec 1;416(2):161-77
The Biochemical journal 2008 Dec 1;416(2):161-77
Adiponectin modulates inflammatory reactions via calreticulin receptor-dependent clearance of early apoptotic bodies.
Takemura Y, Ouchi N, Shibata R, Aprahamian T, Kirber MT, Summer RS, Kihara S, Walsh K
The Journal of clinical investigation 2007 Feb;117(2):375-86
The Journal of clinical investigation 2007 Feb;117(2):375-86
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Adiponectin/Acrp30 in human mesenchymal stem cells differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.