Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB3100 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat Adiponectin/Acrp30 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat Adiponectin/Acrp30 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human or recombinant mouse Adiponectin is observed.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_653345
- Isotype
- IgG
- Antibody clone number
- 861812
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of rat adipose tissue and rat mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adiponectin/Acrp30 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Adiponectin/Acrp30 in Rat Adipocytes. Adiponectin/Acrp30 was detected in Immersion fixed rat mesenchymal stem cells differentiated into adipocytes with Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011) using Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.