Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
Reference
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- Product number
- MAB1063 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1063, RRID:AB_1570958
- Product name
- ADIPOQ monoclonal antibody, clone 1G12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant ADIPOQ.
- Isotype
- IgG
- Antibody clone number
- 1G12
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase.
ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism.
The adipocyte-secreted protein Acrp30 enhances hepatic insulin action.
Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, Yamashita S, Noda M, Kita S, Ueki K, Eto K, Akanuma Y, Froguel P, Foufelle F, Ferre P, Carling D, Kimura S, Nagai R, Kahn BB, Kadowaki T
Nature medicine 2002 Nov;8(11):1288-95
Nature medicine 2002 Nov;8(11):1288-95
ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism.
Berg AH, Combs TP, Scherer PE
Trends in endocrinology and metabolism: TEM 2002 Mar;13(2):84-9
Trends in endocrinology and metabolism: TEM 2002 Mar;13(2):84-9
The adipocyte-secreted protein Acrp30 enhances hepatic insulin action.
Berg AH, Combs TP, Du X, Brownlee M, Scherer PE
Nature medicine 2001 Aug;7(8):947-53
Nature medicine 2001 Aug;7(8):947-53
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of recombinant proteins and mouse liver lysates. To probed with ADIPOQ monoclonal antibody, clone 1G12 (1 : 500) (Cat # MAB1063). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. 1 : Recombinant human ADIPOQ protein (full lenghth). 2 : Recombinant human globular domain of ADIPOQ fusion protein. 3 : Recombinant mouse globular domain of ADIPOQ protein. 4 : Mouse liver lysates. Arrows indicate the oligomer and monomer of ADIPOQ protein.