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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA503913 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503913, RRID:AB_11141750
- Product name
- ADIPOQ (Adiponectin) mouse monoclonal antibody, clone OTI7B11 (formerly 7B11)
- Antibody type
- Monoclonal
- Description
- ADIPOQ (Adiponectin) mouse monoclonal antibody, clone OTI7B11 (formerly 7B11)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ADIPOQ
- Isotype
- IgG
- Antibody clone number
- OTI7B11
- Vial size
- 100 µl
- Concentration
- 0.94 mg/ml
Submitted references Adipocyte-Derived Exosomes Carrying Sonic Hedgehog Mediate M1 Macrophage Polarization-Induced Insulin Resistance via Ptch and PI3K Pathways.
Song M, Han L, Chen FF, Wang D, Wang F, Zhang L, Wang ZH, Zhong M, Tang MX, Zhang W
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(4):1416-1432
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(4):1416-1432
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADIPOQ (RC215161, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADIPO
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-ADIPOQ monoclonal antibody at 1:200.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ADIPOQ mouse monoclonal antibody (TA503913) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ADIPOQ(RC215161).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC215161 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ADIPOQ antibody(TA503913), and then analyzed by flow cytometry.
- Validation comment
- FC
