Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- Ab087545 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Adiponectin Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Adiponectin Antibody, Monoclonal (H2), could be used for WB, IHC and so on. Application: WB: 0.01-2µg/mL IHC: 5-30µg/mL Protein Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 108-244- Antibody clone number
- H2
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
No comments: Submit comment
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Adiponectin Mouse mAb (Ab087545) - Western Blot All lanes: Adiponectin Mouse mAb (Ab087545) at 1/2000 dilution Samples: Lysates at 5 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 28 kDa Exposure time: 50.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Adiponectin Mouse mAb (Ab087545) - IHC DAB staining on IHC Sample: Rat Cardiac Muscle Tissue Primary Ab: 30 µg/mL Adiponectin Mouse mAb (Ab087545) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Adiponectin Mouse mAb (Ab087545) - IHC DAB staining on IHC Sample: Human Cardiac Muscle Tissue Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
