MAB10652

antibody from R&D Systems

Targeting: ADIPOQ ACDC, ACRP30, adiponectin, apM1, GBP28

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB10652

Provider

R&D Systems

Product name

Human Adiponectin/Acrp30 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Adiponectin/Acrp30 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Acrp30 (aa 101-244), recombinant mouse (rm) Acrp30 (aa 111-247), rmAcrp30 (HWM), recombinant rat Acrp30 (aa 1-244), rhC1qTNF1, rmCORS26/C1qTNF3, rhC1qTNF4, rhC1qTNF5, rhC1qTNF9, or rhC1qL2 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q15848


Isotype

IgG

Antibody clone number

553517

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Functional effects of adiponectin on endothelial progenitor cells.

Lavoie V, Kernaleguen AE, Charron G, Farhat N, Cossette M, Mamarbachi AM, Allen BG, Rhéaume E, Tardif JC
Obesity (Silver Spring, Md.) 2011 Apr;19(4):722-8

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of human adipose tissue. PVDF Membrane was probed with 2 µg/mL of Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10652) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Adiponectin/Acrp30 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.