NB100-65810
antibody from Novus Biologicals
Targeting: ADIPOQ
ACDC, ACRP30, adiponectin, apM1, GBP28
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [6]
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Validation data
Reference
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- Product number
- NB100-65810 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65810, RRID:AB_958959
- Product name
- Rabbit Polyclonal Adiponectin/Acrp30 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This recognises an epitope within the C-terminal region (CT) of the 244 amino acid protein Adiponectin.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nucleus-localized adiponectin is survival gatekeeper through miR-214-mediated AIFM2 regulation.
Possible Involvement of Adiponectin, the Anti-Diabetes Molecule, in the Pathogenesis of Alzheimer's Disease.
The amount of C1q-adiponectin complex is higher in the serum and the complex localizes to perivascular areas of fat tissues and the intimal-medial layer of blood vessels of coronary artery disease patients.
Clinical significance of adiponectin expression in colon cancer patients.
Signalling role of adipose tissue: adipokines and inflammation in obesity.
Adiponectin: action, regulation and association to insulin sensitivity.
A family of Acrp30/adiponectin structural and functional paralogs.
Cho J, Teshigawara R, Kameda M, Yamaguchi S, Tada T
Genes to cells : devoted to molecular & cellular mechanisms 2019 Feb;24(2):126-138
Genes to cells : devoted to molecular & cellular mechanisms 2019 Feb;24(2):126-138
Possible Involvement of Adiponectin, the Anti-Diabetes Molecule, in the Pathogenesis of Alzheimer's Disease.
Waragai M, Adame A, Trinh I, Sekiyama K, Takamatsu Y, Une K, Masliah E, Hashimoto M
Journal of Alzheimer's disease : JAD 2016 Apr 8;52(4):1453-9
Journal of Alzheimer's disease : JAD 2016 Apr 8;52(4):1453-9
The amount of C1q-adiponectin complex is higher in the serum and the complex localizes to perivascular areas of fat tissues and the intimal-medial layer of blood vessels of coronary artery disease patients.
Hong ES, Lim C, Choi HY, Ku EJ, Kim KM, Moon JH, Lim S, Park KS, Jang HC, Choi SH
Cardiovascular diabetology 2015 May 9;14:50
Cardiovascular diabetology 2015 May 9;14:50
Clinical significance of adiponectin expression in colon cancer patients.
Canhoroz M, Kanat O, Saraydaroglu O, Buluc E, Avci N, Cubukcu E, Olmez OF, Manavoglu O
Journal of cancer research and therapeutics 2014 Apr-Jun;10(2):347-53
Journal of cancer research and therapeutics 2014 Apr-Jun;10(2):347-53
Signalling role of adipose tissue: adipokines and inflammation in obesity.
Trayhurn P, Wood IS
Biochemical Society transactions 2005 Nov;33(Pt 5):1078-81
Biochemical Society transactions 2005 Nov;33(Pt 5):1078-81
Adiponectin: action, regulation and association to insulin sensitivity.
Lihn AS, Pedersen SB, Richelsen B
Obesity reviews : an official journal of the International Association for the Study of Obesity 2005 Feb;6(1):13-21
Obesity reviews : an official journal of the International Association for the Study of Obesity 2005 Feb;6(1):13-21
A family of Acrp30/adiponectin structural and functional paralogs.
Wong GW, Wang J, Hug C, Tsao TS, Lodish HF
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 13;101(28):10302-7
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 13;101(28):10302-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Adiponectin/Acrp30 Antibody [NB100-65810] - Western blot analysis of Adoponectin in 1. human liver 2. rat liver 3. HL60
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NB100-65810] - IHC analysis of Adiponectin in mouse liver showing nuclear and cytoplasmic staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NB100-65810] - Immunohistochemical staining of rat brain with Rabbit anti Human adiponectin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody [NB100-65810] - Co-localization of C1q and APN deposited in adipose tissue and blood vessels (x100). Positive staining of C1q (red) and APN (green) is shown along the perivascular areas of fat tissues and intimal-medial layer of the blood vessel. C1q and APN co-localized almost completely in the same areas. H&E, hematoxylin and eosin. Image collected and cropped by CiteAb from the following publication (http://www.cardiab.com/content/14/1/50), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - An intracellular stain was performed on Jurkat cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - Analysis of Alexa Fluor 647 conjugate of NB100-65810. An intracellular stain was performed on Jurkat cells with Adiponectin antibody NB100-65810AF647 (blue) and a matched isotype control NBP2-24893AF647 (orange). Cells were fixed with 4% PFA and then perm
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - Analysis of Allophycocyanin conjugate of NB100-65810. An intracellular stain was performed on Jurkat cells with Adiponectin antibody NB100-65810APC (blue) and a matched isotype control NBP2-24893APC (orange). Cells were fixed with 4% PFA and then permeabl
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Adiponectin/Acrp30 Antibody [NB100-65810] - Analysis of Allophycocyanin conjugate of NB100-65810. An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810APC (blue) and a matched isotype control NBP2-24893APC (orange). Cells were fixed with 4% PFA and then permeabliz
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Adiponectin/Acrp30 Antibody [NB100-65810] - An intracellular stain was performed on Jurkat cells with Adiponectin/Acrp30 Antibody NB100-65810AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to AF647.