NB100-56740

antibody from Novus Biologicals

Targeting: TRAF3IP2 ACT1, C6orf2, C6orf4, C6orf5, C6orf6, CIKS, DKFZP586G0522

Provider product page for NB100-56740

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [10]
Comments [0]
Validations
Western blot [3]
Immunohistochemistry [1]

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Product number: NB100-56740 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB100-56740, RRID:AB_2241090
Product name: Rabbit Polyclonal TRAF3IP2 Antibody
Antibody type: Polyclonal
Description: Protein G purified. An approx. 60 kDa band is observed.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 0.1 mg
Concentration: 1.0 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

TRAF3IP2 protein structure - NB100-56740 shown in red.

Submitted references Empagliflozin reduces high glucose-induced oxidative stress and miR-21-dependent TRAF3IP2 induction and RECK suppression, and inhibits human renal proximal tubular epithelial cell migration and epithelial-to-mesenchymal transition.

Das NA, Carpenter AJ, Belenchia A, Aroor AR, Noda M, Siebenlist U, Chandrasekar B, DeMarco VG
Cellular signalling 2020 Apr;68:109506

TRAF3IP2 mediates high glucose-induced endothelin-1 production as well as endothelin-1-induced inflammation in endothelial cells.

Padilla J, Carpenter AJ, Das NA, Kandikattu HK, López-Ongil S, Martinez-Lemus LA, Siebenlist U, DeMarco VG, Chandrasekar B
American journal of physiology. Heart and circulatory physiology 2018 Jan 1;314(1):H52-H64

TRAF3IP2 mediates TWEAK/TWEAKR-induced pro-fibrotic responses in cultured cardiac fibroblasts and the heart.

Das NA, Carpenter AJ, Yoshida T, Kumar SA, Gautam S, Mostany R, Izadpanah R, Kumar A, Mummidi S, Siebenlist U, Chandrasekar B
Journal of molecular and cellular cardiology 2018 Aug;121:107-123

Metformin inhibits aldosterone-induced cardiac fibroblast activation, migration and proliferation in vitro, and reverses aldosterone+salt-induced cardiac fibrosis in vivo.

Mummidi S, Das NA, Carpenter AJ, Kandikattu H, Krenz M, Siebenlist U, Valente AJ, Chandrasekar B
Journal of molecular and cellular cardiology 2016 Sep;98:95-102

Cardiac-restricted Overexpression of TRAF3 Interacting Protein 2 (TRAF3IP2) Results in Spontaneous Development of Myocardial Hypertrophy, Fibrosis, and Dysfunction.

Yariswamy M, Yoshida T, Valente AJ, Kandikattu HK, Sakamuri SS, Siddesha JM, Sukhanov S, Saifudeen Z, Ma L, Siebenlist U, Gardner JD, Chandrasekar B
The Journal of biological chemistry 2016 Sep 9;291(37):19425-36

TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis.

Sakamuri SS, Valente AJ, Siddesha JM, Delafontaine P, Siebenlist U, Gardner JD, Bysani C
Molecular and cellular endocrinology 2016 Jul 5;429:84-92

Aldosterone-induced cardiomyocyte growth, and fibroblast migration and proliferation are mediated by TRAF3IP2.

Somanna NK, Yariswamy M, Garagliano JM, Siebenlist U, Mummidi S, Valente AJ, Chandrasekar B
Cellular signalling 2015 Oct;27(10):1928-38

OxLDL induces endothelial dysfunction and death via TRAF3IP2: inhibition by HDL3 and AMPK activators.

Valente AJ, Irimpen AM, Siebenlist U, Chandrasekar B
Free radical biology & medicine 2014 May;70:117-28

Interleukin-18 enhances IL-18R/Nox1 binding, and mediates TRAF3IP2-dependent smooth muscle cell migration. Inhibition by simvastatin.

Valente AJ, Yoshida T, Izadpanah R, Delafontaine P, Siebenlist U, Chandrasekar B
Cellular signalling 2013 Jun;25(6):1447-56

Advanced oxidation protein products induce cardiomyocyte death via Nox2/Rac1/superoxide-dependent TRAF3IP2/JNK signaling.

Valente AJ, Yoshida T, Clark RA, Delafontaine P, Siebenlist U, Chandrasekar B
Free radical biology & medicine 2013 Jul;60:125-35

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: TRAF3IP2 Antibody [NB100-56740] - Simple Western lane view shows a specific band for TRAF3IP2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of ACT1 in human kidney lysate in the A) absence and B) presence of immunizing peptide using ACT1 antibody at 2 ug/ml.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of CIKS using NB100-56740 on 15 ugs of mouse kidney cell lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: TRAF3IP2 Antibody [NB100-56740] - Analysis of ACT1 in human kidney tissue using ACT1 antibody at 5 ug/ml.