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- Antigen structure
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- Product number
- PA5-99891 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR125 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total GPR125.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references GPR125 positively regulates osteoclastogenesis potentially through AKT-NF-κB and MAPK signaling pathways.
Tang CY, Wang H, Zhang Y, Wang Z, Zhu G, McVicar A, Li YP, Chen W
International journal of biological sciences 2022;18(6):2392-2405
International journal of biological sciences 2022;18(6):2392-2405
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPR125 in HUVEC cell lysate. Samples were incubated with GPR125 polyclonal antibody (Product # PA5-99891).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Gpr125 impairs osteoclast differentiation and function. (A-G) MBM was transduced by sh-SC, sh-GPR125 lentivirus, or mock control and stimulated by M-CSF (10ng/mL) and RANKL (10ng/mL). (A) When cells were induced by M-CSF/RANKL for 5 days, cells were stained for TRAP activity at a 12-well-plate. Scale bar = 200mum. (B) To detect the acid secretion function, cells were performed with Acridine Orange (AO) stain in a 24-well-plate when induced by M-CSF/ RANKL for 5 days. Scale bar = 200mum. (C) To determine the bone resorption activity of mature osteoclasts, cells of three groups were stained with WGA reagent. Scale bar = 100mum. (D) Quantification of A-C . (E) Western blot analysis of osteoclasts from three groups on Day 5 to confirm the knockdown of Gpr125 . (F) Quantification of protein levels of Gpr125 in E normalized to Gapdh. (G) Western blot analysis of osteoclasts from three groups on Day 5 to explore the influence of the knockdown of Gpr125 on the expression of osteoclast marker protein Pu.1. (H) Quantification of protein levels of Gpr125 and Pu.1 in G normalized to Gapdh. Data is expressed as mean +- SEM, one dot represents one sample (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.
